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1 ARTICLES PUBLISHED IN JoVE

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Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Riccardo Balzan¹, Laetitia Fernandes¹, Arnaud Comment², Laetitia Pidial³, Bertrand Tavitian³, Paul R. Vasos¹

¹LCBPT - UMR8601, Institut de Chimie, Université Paris Descartes, ²Institute of Physics of Biological Systems, EPFL, ³PARCC - INSERM U970, Université Paris Descartes

The sensitivity enhancement provided by dissolution dynamic nuclear polarization (DNP) enables following metabolic processes in real time by NMR and MRI. The characteristics and performances of a dedicated dissolution DNP setup designed for study enzymatic reactions are discussed.