Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMRRiccardo Balzan 1, Laetitia Fernandes 1, Arnaud Comment 2, Laetitia Pidial 3, Bertrand Tavitian 3, Paul R. Vasos 1
1LCBPT - UMR8601, Institut de Chimie, Université Paris Descartes, 2Institute of Physics of Biological Systems, EPFL, 3PARCC - INSERM U970, Université Paris Descartes
The sensitivity enhancement provided by dissolution dynamic nuclear polarization (DNP) enables following metabolic processes in real time by NMR and MRI. The characteristics and performances of a dedicated dissolution DNP setup designed for study enzymatic reactions are discussed.
