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Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris Descartes

1 ARTICLES PUBLISHED IN JoVE

Biology

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli

Aude G. Bernheim^*1, Vincent K. Libis^*1, Ariel B. Lindner¹, Edwin H. Wintermute¹

¹U1001, Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris Descartes

We describe a method to knock down gene expression in a growing population of E. coli cells using sequence-targeted sRNA expression cassettes delivered by an M13 phagemid vector.