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Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris Descartes

1 ARTICLES PUBLISHED IN JoVE

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Biology

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli
Aude G. Bernheim *1, Vincent K. Libis *1, Ariel B. Lindner 1, Edwin H. Wintermute 1
1U1001, Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris Descartes

We describe a method to knock down gene expression in a growing population of E. coli cells using sequence-targeted sRNA expression cassettes delivered by an M13 phagemid vector.

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