Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic InjectionCatherine J. Fricano-Kugler 1, Michael R. Williams 1, Julia R. Salinaro 1, Meijie Li 1, Bryan Luikart 1
1Department of Physiology and Neurobiology, Geisel School of Medicine at Dartmouth College
The CRISPR/Cas9 system offers the potential to make targeted genome editing accessible and affordable to the scientific community. This protocol is intended to demonstrate how to create viruses that will knockout a gene of interest using the CRISPR/Cas9 system, and then inject them stereotaxically into the adult mouse brain.
