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School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), UNIST-gil 50, Ulsan, Republic of Korea

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Yang-Seok Park¹, Vijaya Sunkara¹, Yubin Kim¹, Won Seok Lee^1,2, Ja-Ryoung Han^1,3, Yoon-Kyoung Cho^1,4

¹Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), UNIST-gil 50, Ulsan, Republic of Korea, ²Agency for Defense Development (ADD), Daejeon, Republic of Korea, ³KOGAS (Korea Gas Corporation) Research Institute, ⁴Center for Soft and Living Matter, Institute for Basic Science (IBS)

This protocol demonstrates how to achieve femto molar detection sensitivity of proteins in 10 µL of whole blood within 30 min. This can be achieved by using electrospun nanofibrous mats integrated in a lab-on-a-disc, which offers high surface area as well as effective mixing and washing for enhanced signal-to-noise ratio.

Cancer Research

Culture, Manipulation, and Orthotopic Transplantation of Mouse Bladder Tumor Organoids

Yubin Kim^*1, Juhee Lee^*1, SungEun Kim^*1, Kunyoo Shin¹

¹Department of Life Sciences, Pohang University of Science and Technology

This protocol provides detailed experimental steps to establish a three-dimensional in vitro culture of bladder tumor organoids derived from carcinogen-induced murine bladder cancer. Culture methods including passaging, genetic engineering, and orthotopic transplantation of tumor organoids are described.

JoVE Journal

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Chi-Ju Kim^*1,2, Morgan D. Kuczler^*1, Liang Dong^1,3, Junyoung Kim^2,4, Sarah R. Amend¹, Yoon-Kyoung Cho^2,4, Kenneth J. Pienta¹

¹The Brady Urological Institute, Johns Hopkins University School of Medicine, ²Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), ³Department of Urology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, ⁴Center for Soft and Living Matter, Institute for Basic Science (IBS)

Extracellular vesicles (EVs) contribute to cellular biology and intercellular communications. There is a need for practical assays to visualize and quantify EVs uptake by the cells. The current protocol proposes the EV uptake assay by utilizing three-dimensional fluorescence imaging via confocal microscopy, following EV isolation by a nano-filtration-based microfluidic device.