JoVE Logo
Faculty Resource Center

Sign In

School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), UNIST-gil 50, Ulsan, Republic of Korea

3 ARTICLES PUBLISHED IN JoVE

image

Bioengineering

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood
Yang-Seok Park 1, Vijaya Sunkara 1, Yubin Kim 1, Won Seok Lee 1,2, Ja-Ryoung Han 1,3, Yoon-Kyoung Cho 1,4
1Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), UNIST-gil 50, Ulsan, Republic of Korea, 2Agency for Defense Development (ADD), Daejeon, Republic of Korea, 3KOGAS (Korea Gas Corporation) Research Institute, 4Center for Soft and Living Matter, Institute for Basic Science (IBS)

This protocol demonstrates how to achieve femto molar detection sensitivity of proteins in 10 µL of whole blood within 30 min. This can be achieved by using electrospun nanofibrous mats integrated in a lab-on-a-disc, which offers high surface area as well as effective mixing and washing for enhanced signal-to-noise ratio.

image

Cancer Research

Culture, Manipulation, and Orthotopic Transplantation of Mouse Bladder Tumor Organoids
Yubin Kim *1, Juhee Lee *1, SungEun Kim *1, Kunyoo Shin 1
1Department of Life Sciences, Pohang University of Science and Technology

This protocol provides detailed experimental steps to establish a three-dimensional in vitro culture of bladder tumor organoids derived from carcinogen-induced murine bladder cancer. Culture methods including passaging, genetic engineering, and orthotopic transplantation of tumor organoids are described.

image

JoVE Journal

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis
Chi-Ju Kim *1,2, Morgan D. Kuczler *1, Liang Dong 1,3, Junyoung Kim 2,4, Sarah R. Amend 1, Yoon-Kyoung Cho 2,4, Kenneth J. Pienta 1
1The Brady Urological Institute, Johns Hopkins University School of Medicine, 2Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), 3Department of Urology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 4Center for Soft and Living Matter, Institute for Basic Science (IBS)

Extracellular vesicles (EVs) contribute to cellular biology and intercellular communications. There is a need for practical assays to visualize and quantify EVs uptake by the cells. The current protocol proposes the EV uptake assay by utilizing three-dimensional fluorescence imaging via confocal microscopy, following EV isolation by a nano-filtration-based microfluidic device.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved