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Institute of Genetics and Biophysics "A. Buzzati Traverso", CNR

1 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection

Ombretta Guardiola¹, Gennaro Andolfi¹, Mario Tirone^2,3, Francescopaolo Iavarone¹, Silvia Brunelli², Gabriella Minchiotti¹

¹Stem Cell Fate Laboratory, Institute of Genetics and Biophysics "A. Buzzati Traverso", CNR, ²School of Medicine and Surgery, University of Milano-Bicocca, ³Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute

This manuscript describes a detailed protocol to induce acute skeletal muscle regeneration in adult mice and subsequent manipulations of the muscles, such as dissection, freezing, cutting, routine staining, and myofiber cross-sectional area analysis.