Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin InjectionOmbretta Guardiola 1, Gennaro Andolfi 1, Mario Tirone 2,3, Francescopaolo Iavarone 1, Silvia Brunelli 2, Gabriella Minchiotti 1
1Stem Cell Fate Laboratory, Institute of Genetics and Biophysics "A. Buzzati Traverso", CNR, 2School of Medicine and Surgery, University of Milano-Bicocca, 3Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute
This manuscript describes a detailed protocol to induce acute skeletal muscle regeneration in adult mice and subsequent manipulations of the muscles, such as dissection, freezing, cutting, routine staining, and myofiber cross-sectional area analysis.
