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Guangdong Provincial Engineering Research Center for On-line Source Apportionment System of Air Pollution

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy

Haochuan Zhang^*1,2,3, JunGang Huang^*2,3,4, Xin Liu^2,3, Ping Zhu⁴, Zhongrong Li¹, Xue Li^2,3

¹Department of Pediatric Surgery, The Second Affiliated Hospital & Yuying Children's Hospital, Wenzhou Medical University, ²Departments of Urology, Boston Children's Hospital, ³Department of Surgery, Harvard Medical School, ⁴Department of Cardiovascular Surgery, Guangdong Cardiovascular Institute, Guangdong General Hospital, Guangdong Academy of Medical Sciences

We describe a detailed protocol using high-resolution episcopic microscopy to acquire three-dimensional (3D) images of mouse embryos. This improved protocol utilizes a modified tissue preparation method to enhance penetration of the fluorescent dye, thereby permitting morphometric analysis of both small and large-sized specimens.

Chemistry

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry

Xue Li^*1,2, Dan D. Huang^*3, Rui Du^1,2, Zhi J. Zhang¹, Chak K. Chan³, Zheng X. Huang^1,2, Zhen Zhou^1,2

¹Institute of Mass Spectrometer and Atmospheric Environment, Jinan University, ²Guangdong Provincial Engineering Research Center for On-line Source Apportionment System of Air Pollution, ³School of Energy and Environment, City University of Hong Kong

A protocol for characterizing chemical composition of exhaled breath in real time by using secondary nanoelectrospray ionization coupled to high resolution mass spectrometry is demonstrated.