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Guangdong Provincial Engineering Research Center for On-line Source Apportionment System of Air Pollution

2 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy
Haochuan Zhang *1,2,3, JunGang Huang *2,3,4, Xin Liu 2,3, Ping Zhu 4, Zhongrong Li 1, Xue Li 2,3
1Department of Pediatric Surgery, The Second Affiliated Hospital & Yuying Children's Hospital, Wenzhou Medical University, 2Departments of Urology, Boston Children's Hospital, 3Department of Surgery, Harvard Medical School, 4Department of Cardiovascular Surgery, Guangdong Cardiovascular Institute, Guangdong General Hospital, Guangdong Academy of Medical Sciences

We describe a detailed protocol using high-resolution episcopic microscopy to acquire three-dimensional (3D) images of mouse embryos. This improved protocol utilizes a modified tissue preparation method to enhance penetration of the fluorescent dye, thereby permitting morphometric analysis of both small and large-sized specimens.

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Chemistry

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry
Xue Li *1,2, Dan D. Huang *3, Rui Du 1,2, Zhi J. Zhang 1, Chak K. Chan 3, Zheng X. Huang 1,2, Zhen Zhou 1,2
1Institute of Mass Spectrometer and Atmospheric Environment, Jinan University, 2Guangdong Provincial Engineering Research Center for On-line Source Apportionment System of Air Pollution, 3School of Energy and Environment, City University of Hong Kong

A protocol for characterizing chemical composition of exhaled breath in real time by using secondary nanoelectrospray ionization coupled to high resolution mass spectrometry is demonstrated.

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