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Children's Hospital Colorado

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Transurethral Instillation Procedure in Adult Male Mouse

Sanghee Lee^*1, Alonso Carrasco Jr.^*2, Randall B. Meacham¹, Anna P. Malykhina¹

¹Division of Urology, Department of Surgery, University of Colorado School of Medicine, ²Pediatric Urology, Children's Hospital Colorado

Transurethral instillation is a challenging procedure and has not been well described in the literature. The aim of this manuscript is to describe a technique for transurethral insertion of a catheter for intravesical delivery of liquids with active substances into the urinary bladder and/or prostate in adult male mice.

Neuroscience

Air-Inflation of Murine Lungs with Vascular Perfusion-Fixation

Stacey M. Thomas¹, Joseph Bednarek², William J. Janssen¹, Patrick S. Hume¹

¹Division of Pulmonary, Sleep and Critical Care Medicine, Department of Medicine, National Jewish Health, ²Section of Pediatric Gastroenterology, Hepatology, and Nutrition, Children's Hospital Colorado

Presented is a method for air-inflation with vascular perfusion-fixation of the lungs that preserves the location of cells within airways, alveoli and interstitium for structure-function analyses. Constant airway pressure is maintained with an air-inflation chamber while fixative is perfused via the right ventricle. Lungs are processed for histologic studies.