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First Affiliated Hospital of Nanjing Medical University

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Electrochemiluminescence Assays for Human Islet Autoantibodies

Yong Gu^1,2, Zhiyuan Zhao¹, Dongmei Miao¹, Hilary High¹, Tao Yang², Liping Yu¹

¹Barbara Davis Center for Childhood Diabetes, University of Colorado Denver, ²Department of Endocrinology, First Affiliated Hospital of Nanjing Medical University

Here, we presented the methods to detect human islet autoantibodies using electrochemiluminescence (ECL) assays. The protocol, used to predict type 1 diabetes, can be expanded upon to detect autoantibodies for other autoimmune diseases.

Medicine

A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases

Xiaofan Jia^*1,2, Ling He^*1,3, Yong Gu¹, Hilary High¹, Liping Yu¹

¹Barbara Davis Center for Diabetes, University of Colorado School of Medicine, ²Department of Endocrinology, Beijing Hospital, National Center of Gerontology; Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, ³Department of endocrinology, Guangzhou First People's Hospital, School of Medicine, South China University of Technology

We model a simple multiplexed ECL assay that combines 7 autoantibody assays together. The assay is capable of screening for T1D and multiple other autoimmune diseases, simultaneously, including celiac disease, autoimmune thyroid disease, and autoimmune polyglandular syndrome 1.