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Guangzhou Institutes of Biomedicine and Health and Guangzhou Medical University

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

A Familial Hypercholesterolemia Human Liver Chimeric Mouse Model Using Induced Pluripotent Stem Cell-derived Hepatocytes

Jiayin Yang^*1,2,6, Lai-Yung Wong^*2, Xiao-Yu Tian^*3, Rui Wei^1,2, Wing-Hon Lai², Ka-Wing Au², Zhiwei Luo^4,5, Carl Ward^4,5, Wai-In Ho², David P. Ibañez^4,5, Hao Liu^4,5, Xichen Bao^4,5, Baoming Qin^4,5, Yu Huang³, Miguel A. Esteban^4,5,7, Hung-Fat Tse^1,2,6,7

¹Department of Medicine, University of Hong Kong-Shenzhen Hospital, ²The Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, University of Hong Kong, ³School of Biomedical Sciences, Institute of Vascular Medicine, Li Ka Shing Institute of Health Sciences, Chinese University of Hong Kong, ⁴Key Laboratory of Regenerative Biology of the Chinese Academy of Sciences, Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health and Guangzhou Medical University, ⁵Laboratory of RNA, Chromatin, and Human Disease, CAS Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, ⁶Research Centre of Heart, Brain, Hormone, and Healthy Ageing, Li Ka Shing Faculty of Medicine, University of Hong Kong, ⁷Hong Kong-Guangdong Stem Cell and Regenerative Medicine Research Centre, University of Hong Kong and Guangzhou Institutes of Biomedicine and Health

Here, we present a protocol to generate a human liver chimeric mouse model of familial hypercholesterolemia using human induced pluripotent stem cell-derived hepatocytes. This is a valuable model for testing new therapies for hypercholesterolemia.

Behavior

Analysis of Learning and Memory Ability in an Alzheimer's Disease Mouse Model using the Morris Water Maze

Huiling Tian¹, Ning Ding², Mengwei Guo¹, Shun Wang¹, Zidong Wang¹, Hao Liu¹, Jiayi Yang¹, Yujie Li³, Jingyu Ren¹, Jing Jiang⁴, Zhigang Li¹

¹School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, ²Guang'anmen Hospital of China Academy of Chinese Medical Science, ³The Third Affiliated Hospital of Beijng University of Chinese Medicine, ⁴School of Nursing, Beijing University of Chinese Medicine

Herein, a protocol to conduct the Morris water maze tests to evaluate the ability of learning and memory of Alzheimer’s Disease model mice and to assess the effect of manual acupuncture for treating them is described.