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University Hospital of Nice

2 ARTICLES PUBLISHED IN JoVE

Medicine

Database-guided Flow-cytometry for Evaluation of Bone Marrow Myeloid Cell Maturation

Carmen Mariana Aanei¹, Marie-Christine Jacob², Richard Veyrat-Masson³, Tiphanie Picot¹, Maria Alessandra Rosenthal-Allieri⁴, Anne-Catherine Lhoumeau⁵, Michel Ticchioni⁴, Florent Dumezy⁶, Lydia Campos Catafal¹

¹Department of Hematology, University Hospital of Saint-Etienne, ²Department of Immunology, University Hospital of Grenoble-Alpes, ³Department Hematology, University Hospital of Clermont-Ferrand, ⁴Department of Immunology, University Hospital of Nice, ⁵Department of Biopathology, Institute Paoli-Calmettes, ⁶Department of Hematology, University Hospital of Lille

The MDS diagnosis is difficult in the absence of morphological criteria or non-informative cytogenetics. MFC could help refine the MDS diagnostic process. To become useful for clinical practice, the MFC analysis must be based on parameters with sufficient specificity and sensitivity, and data should be reproducible between different operators.

Medicine

Vascularized Composite Hand Allograft Procurement and Preparation for Distal and Proximal Forearm Allotransplantation: A Stepwise Approach

Tanguy Perraudin¹, Yanis Berkane², Antoine Sicard^3,4, Nicolas Bronsard⁵, Olivier Camuzard¹, Elise Lupon^1,3

¹Department of Plastic and Reconstructive Surgery, Institut Universitaire Locomoteur et du Sport, Pasteur 2 Hospital, University Côte d'Azur, ²Department of Plastic, Reconstructive and Aesthetic Surgery, Hospital Sud, University of Rennes 1, ³Laboratory of Molecular PhysioMedicine (LP2M), UMR 7370, CNRS, University Côte d'Azur, ⁴Department of Nephrology, Dialysis and Kidney Transplantation, University Hospital of Nice, ⁵Department of orthopeadic Surgery, Institut Universitaire Locomoteur et du Sport, Pasteur 2 Hospital, University Côte d'Azur

Here, we present a protocol for procuring and preparing vascularized composite hand allografts during distal or proximal forearm transplantation.