An Orbital Shaking Culture of Mammalian Cells in O-shaped Vessels to Produce Uniform AggregatesIkki Horiguchi 1,2, Ikumi Suzuki 3, Takashi Morimura 3, Yasuyuki Sakai 1,4
1Department of Chemical System Engineering, University of Tokyo, 2Department of Biotechnology, Osaka University, 3Biotech Business Unit, Fukoku Co. Ltd, 4Institute of Industrial Science, University of Tokyo
Here we present a protocol for using O-shaped vessels, specialized for suspension cultures of cellular aggregates, with orbital shaking. The HEK293 cells grown in this bag form more homogeneous aggregates than those grown in conventional culture vessels.
