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Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences (CAS)

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

Synthesis and Characterization of Placental Chondroitin Sulfate A (plCSA)-Targeting Lipid-Polymer Nanoparticles

Baozhen Zhang^*1, Mingbin Zheng^*2,3, Lintao Cai², Xiujun Fan¹

¹Laboratory for Reproductive Health, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences (CAS), ²Guangdong Key Laboratory of Nanomedicine, CAS Key Lab for Health Informatics, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences (CAS), ³Department of Chemistry, Guangdong Medical University

Here, we present a protocol for the synthesis of placental chondroitin sulfate A binding peptide (plCSA-BP)-conjugated lipid-polymer nanoparticles via single-step sonication and bioconjugate techniques. These particles constitute a novel tool for the targeted delivery of therapeutics to most human tumors and placental trophoblasts to treat cancers and placental disorders.

Bioengineering

Comprehensive Evaluation of the Effectiveness and Safety of Placenta-Targeted Drug Delivery Using Three Complementary Methods

Baozhen Zhang¹, Zhilong Chen^1,2, Jinyu Han^1,3, Mengxia Li¹, Nihar R. Nayak⁴, Xiujun Fan¹

¹Laboratory for Reproductive Health, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, ²College of Veterinary Medicine, Hunan Agricultural University, ³Key Laboratory of Chemical Engineering Process and Technology for High-efficiency Conversion, College of Chemistry and Material Sciences, Heilongjiang University, ⁴Department of Obstetrics and Gynecology, Wayne State University School of Medicine

We describe a system that utilizes three methods to evaluate the safety and effectiveness of placenta-targeted drug delivery: in vivo imaging to monitor nanoparticle accumulation, high-frequency ultrasound to monitor placental and fetal development, and HPLC to quantify drug delivery to tissue.