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Center for Systems Biology Dresden

2 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development
Jaroslav Icha *1, Christopher Schmied *1, Jaydeep Sidhaye 1, Pavel Tomancak 1, Stephan Preibisch 1,2,3, Caren Norden 1
1Max Planck Institute of Molecular Cell Biology and Genetics, 2HHMI Janelia Research Campus, 3Berlin Institute of Medical Systems Biology of the Max Delbrück Center

Light sheet fluorescence microscopy is an excellent tool for imaging embryonic development. It allows recording of long time-lapse movies of live embryos in near physiological conditions. We demonstrate its application for imaging zebrafish eye development across wide spatio-temporal scales and present a pipeline for fusion and deconvolution of multiview datasets.

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Developmental Biology

Analysis of Actomyosin Dynamics at Local Cellular and Tissue Scales Using Time-lapse Movies of Cultured Drosophila Egg Chambers
Ivana Viktorinová 1,2, Robert Haase *1,2, Tobias Pietzsch *1,2, Ian Henry 1,2, Pavel Tomancak 1,2
1Max Planck Institute of Molecular Cell Biology and Genetics, 2Center for Systems Biology Dresden

This protocol provides a Fiji-based, user-friendly methodology along with straightforward instructions explaining how to reliably analyze actomyosin behavior in individual cells and curved epithelial tissues. No programming skills are required to follow the tutorial; all steps are performed in a semi-interactive manner using the graphical user interface of Fiji and associated plugins.

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