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Center for Systems Biology Dresden

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development

Jaroslav Icha^*1, Christopher Schmied^*1, Jaydeep Sidhaye¹, Pavel Tomancak¹, Stephan Preibisch^1,2,3, Caren Norden¹

¹Max Planck Institute of Molecular Cell Biology and Genetics, ²HHMI Janelia Research Campus, ³Berlin Institute of Medical Systems Biology of the Max Delbrück Center

Light sheet fluorescence microscopy is an excellent tool for imaging embryonic development. It allows recording of long time-lapse movies of live embryos in near physiological conditions. We demonstrate its application for imaging zebrafish eye development across wide spatio-temporal scales and present a pipeline for fusion and deconvolution of multiview datasets.

Developmental Biology

Analysis of Actomyosin Dynamics at Local Cellular and Tissue Scales Using Time-lapse Movies of Cultured Drosophila Egg Chambers

Ivana Viktorinová^1,2, Robert Haase^*1,2, Tobias Pietzsch^*1,2, Ian Henry^1,2, Pavel Tomancak^1,2

¹Max Planck Institute of Molecular Cell Biology and Genetics, ²Center for Systems Biology Dresden

This protocol provides a Fiji-based, user-friendly methodology along with straightforward instructions explaining how to reliably analyze actomyosin behavior in individual cells and curved epithelial tissues. No programming skills are required to follow the tutorial; all steps are performed in a semi-interactive manner using the graphical user interface of Fiji and associated plugins.