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Kobe University Graduate School of Medicine

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants

Sota Iwatani¹, Makiko Yoshida², Keiji Yamana¹, Daisuke Kurokawa¹, Jumpei Kuroda¹, Khin Kyae Mon Thwin¹, Suguru Uemura¹, Satoru Takafuji¹, Nanako Nino¹, Tsubasa Koda, Masami Mizobuchi⁴, Masahiro Nishiyama¹, Kazumichi Fujioka¹, Hiroaki Nagase¹, Ichiro Morioka⁵, Kazumoto Iijima¹, Noriyuki Nishimura¹

¹Department of Pediatrics, Kobe University Graduate School of Medicine, ²Department of Pathology, Kobe Children's Hospital, ³Department of Pediatrics, Hyogo College of Medicine, ⁴Department of Developmental Pediatrics, Shizuoka Children's Hospital, ⁵Department of Pediatrics, Nihon University School of Medicine

Human umbilical cord (UC) can be obtained during the perinatal period as a result of preterm, term, and postterm delivery. In this protocol, we describe the isolation and characterization of UC-derived mesenchymal stem cells (UC-MSCs) from fetuses/infants at 19-40 weeks of gestation.

Biology

Isolation Method for Long-Term and Short-Term Hematopoietic Stem Cells

Katsuyuki Nishi^1,2, Akiomi Nagasaka^1,2, Taro Sakamaki^1,2, Kay Sadaoka^1,2, Masanori Miyanishi^1,2

¹Hematopoietic Stem Cell Biology and Medical Innovation (HSCBMI), Department of Pediatrics, Kobe University Graduate School of Medicine, ²RIKEN Center for Biosystems Dynamics Research

We present a step-by-step protocol for the isolation of long-term hematopoietic stem cells (LT-HSCs) and short-term HSCs (ST-HSCs) using the Hoxb5 reporter system.