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Graduate School of Medicine, Gunma University

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting

Paul Turko¹, Keenan Groberman¹, Toralf Kaiser², Yuchio Yanagawa³, Imre Vida¹

¹Institut für Integrative Neuroanatomie, Charité, Universitätsmedizin Berlin, ²Department for Cytometry and Cellsorting, German Rheumatism Research Center, Leibniz Institute, ³Departments of Genetic and Behavioral Neuroscience, Graduate School of Medicine, Gunma University

This protocol describes a cell sorting based method for the purification and culture of fluorescent GABAergic or glutamatergic neurons from the neocortex and hippocampus of postnatal mice or rats.