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Beckman Research Institute at the City of Hope

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery

Jiehua Zhou¹, Haitang Li¹, Jane Zhang², Swiderski Piotr³, John Rossi¹

¹Department of Molecular and Cellular Biology, Beckman Research Institute of City of Hope, ²Graduate School of Biological Sciences, Beckman Research Institute of City of Hope, ³Shared Resource-DNA/RNA Peptide, Beckman Research Institute of City of Hope

Several 2’-Fluoro RNA aptamers against HIV-1Ba-L gp120 with nanomole affinity are isolated from a RNA library by in vitro SELEX procedure. A new dual inhibitory function anti-gp120 aptamer-siRNA chimera is created and shows considerable promise for systemic anti-HIV therapy.

Immunology and Infection

Humanized NOD/SCID/IL2rγ^null (hu-NSG) Mouse Model for HIV Replication and Latency Studies

Xin Xia¹, Haitang Li¹, Sangeetha Satheesan^1,2, Jiehua Zhou¹, John J. Rossi¹

¹Department of Molecular and Cellular Biology, Beckman Research Institute of City of Hope, ²Irell and Manela Graduate School of Biological Sciences, Beckman Research Institute of City of Hope

This protocol provides a method to establish humanized mice (hu-NSG) via intrahepatic injection of human hematopoietic stem cells into radiation-conditioned neonatal NSG mice. The hu-NSG mouse is susceptible to HIV infection and combinatorial antiretroviral therapy (cART) and serves as a suitable pathophysiological model for HIV replication and latency investigations.

Immunology and Infection

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry

Jerlisa Ann C. Arizala^1,2, Pritsana Chomchan¹, Haitang Li¹, Roger Moore³, Helen Ge³, Dominique L. Ouellet¹, John J. Rossi^1,2

¹Molecular and Cellular Biology Department, Beckman Research Institute at the City of Hope, ²Irell & Manella Graduate School of Biological Sciences, ³Department of Molecular Immunology, Beckman Research Institute and the City of Hope

Here we describe Rev immunoprecipitation in the presence of HIV-1 replication for mass spectrometry. The methods described can be used for the identification of nucleolar factors involved in the HIV-1 infectious cycle and are applicable to other disease models for the characterization of understudied pathways.