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Beckman Research Institute at the City of Hope

3 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery
Jiehua Zhou 1, Haitang Li 1, Jane Zhang 2, Swiderski Piotr 3, John Rossi 1
1Department of Molecular and Cellular Biology, Beckman Research Institute of City of Hope, 2Graduate School of Biological Sciences, Beckman Research Institute of City of Hope, 3Shared Resource-DNA/RNA Peptide, Beckman Research Institute of City of Hope

Several 2’-Fluoro RNA aptamers against HIV-1Ba-L gp120 with nanomole affinity are isolated from a RNA library by in vitro SELEX procedure. A new dual inhibitory function anti-gp120 aptamer-siRNA chimera is created and shows considerable promise for systemic anti-HIV therapy.

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Immunology and Infection

Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies
Xin Xia 1, Haitang Li 1, Sangeetha Satheesan 1,2, Jiehua Zhou 1, John J. Rossi 1
1Department of Molecular and Cellular Biology, Beckman Research Institute of City of Hope, 2Irell and Manela Graduate School of Biological Sciences, Beckman Research Institute of City of Hope

This protocol provides a method to establish humanized mice (hu-NSG) via intrahepatic injection of human hematopoietic stem cells into radiation-conditioned neonatal NSG mice. The hu-NSG mouse is susceptible to HIV infection and combinatorial antiretroviral therapy (cART) and serves as a suitable pathophysiological model for HIV replication and latency investigations.

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Immunology and Infection

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry
Jerlisa Ann C. Arizala 1,2, Pritsana Chomchan 1, Haitang Li 1, Roger Moore 3, Helen Ge 3, Dominique L. Ouellet 1, John J. Rossi 1,2
1Molecular and Cellular Biology Department, Beckman Research Institute at the City of Hope, 2Irell & Manella Graduate School of Biological Sciences, 3Department of Molecular Immunology, Beckman Research Institute and the City of Hope

Here we describe Rev immunoprecipitation in the presence of HIV-1 replication for mass spectrometry. The methods described can be used for the identification of nucleolar factors involved in the HIV-1 infectious cycle and are applicable to other disease models for the characterization of understudied pathways.

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