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G.EN.E.R.A. Centers for Reproductive Medicine

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Human Blastocyst Biopsy and Vitrification

Roberta Maggiulli¹, Adriano Giancani^1,2, Danilo Cimadomo¹, Filippo M. Ubaldi¹, Laura Rienzi¹

¹G.EN.E.R.A. Centers for Reproductive Medicine, ²DAHFMO, Unit of Histology and Medical Embryology, Sapienza University of Rome

Blastocyst biopsy and vitrification are required to efficiently conduct preimplantation genetic testing. An approach entailing the sequential opening of the zona pellucida and retrieval of 7-8 trophectoderm cells in day 5-7 post-insemination limits both the number of manipulations required and the exposure of the embryo to sub-optimal environmental conditions.

Medicine

Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives

Roberta Maggiulli¹, Alberto Vaiarelli¹, Danilo Cimadomo¹, Adriano Giancani¹, Luisa Tacconi¹, Gemma Fabozzi¹, Filippo Maria Ubaldi¹, Laura Rienzi¹

¹Clinica Valle Giulia, GeneraLife IVF

Oocyte cryopreservation is recognized by several international scientific societies as the gold standard for fertility preservation in postpubertal women. Appropriate clinical and laboratory strategies ensure maximum efficacy, efficiency, and safety of fertility preservation treatments.