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Université Joseph Fourier (Grenoble 1)

1 ARTICLES PUBLISHED IN JoVE

Bioengineering

Traction Force Microscopy to Study B Lymphocyte Activation

Anita Kumari¹, Judith Pineau¹, Ana-Maria Lennon-Duménil¹, Martial Balland², Paolo Pierobon¹

¹Institut Curie, PSL Research University, INSERM U932, ²Laboratoire Interdisciplinaire de Physique, Université Joseph Fourier (Grenoble 1)

Here we present a protocol used to perform traction force microscopy experiments on B cells. We describe the preparation of soft polyacrylamide gels and their functionalization, as well as data acquisition at the microscope and a summary of data analysis.