Elimination of Serotonergic Neurons by Stereotaxic Injection of 5,7-Dihydroxytryptamine in the Dorsal Raphe Nuclei of MiceLining Cao 1, Zhiqiang Zhang 1, Xinyu Lu 1, Guanhao Wang 1, Du Meng 1, Chang Liu 1, Ji Yun 1, Ting Xu 1, Chun Zhao 2, Jianfeng Lu 1
1Institute for Regenerative Medicine, Shanghai East Hospital, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, 2Department of Ophthalmology, Shanghai Tenth People's Hospital Affiliated to Tongji University School of Medicine
This protocol describes a dorsal raphe nucleus (DRN)-lesioned mouse model (>90% survival rate in experimental mice) with stable loss of dorsal raphe serotonergic neurons by stereotaxic injection of 5,7-dihydroxytryptamine into the DRN using an angled approach to prevent injury to the superior sagittal sinus.
