Shanghai Tenth People's Hospital Affiliated to Tongji University School of Medicine

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Elimination of Serotonergic Neurons by Stereotaxic Injection of 5,7-Dihydroxytryptamine in the Dorsal Raphe Nuclei of Mice

Lining Cao¹, Zhiqiang Zhang¹, Xinyu Lu¹, Guanhao Wang¹, Du Meng¹, Chang Liu¹, Ji Yun¹, Ting Xu¹, Chun Zhao², Jianfeng Lu¹

¹Institute for Regenerative Medicine, Shanghai East Hospital, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, ²Department of Ophthalmology, Shanghai Tenth People's Hospital Affiliated to Tongji University School of Medicine

This protocol describes a dorsal raphe nucleus (DRN)-lesioned mouse model (>90% survival rate in experimental mice) with stable loss of dorsal raphe serotonergic neurons by stereotaxic injection of 5,7-dihydroxytryptamine into the DRN using an angled approach to prevent injury to the superior sagittal sinus.