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General Autority - City of Scientific Researches and Technological Applications

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells

Xiaoyue Sun^*1,2,3, Xiaoqiang Yang^*1,2,3,4, Chui Zeng^1,2,3, Fayed Attia Koutb Megahed^1,2,3,5, Qi Zhou^1,2,3, Tingdang Liu^1,2,3, Waqas Iqbal^1,2,3, Pingnan Sun^1,2,3, Xiaoling Zhou^1,2,3

¹Stem Cell Research Center, Shantou University Medical College, ²The Center for Reproductive Medicine, Shantou University Medical College, ³Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College, ⁴Medical Research Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, ⁵Department of Nucleic Acid Researches, Genetic Engineering and Biotechnology Research Institute, General Autority - City of Scientific Researches and Technological Applications

This manuscript describes a detailed protocol for Hepatitis B virus (HBV) infection in novel engineered 293T cells (293T-NE-3NRs, expressing human NTCP, HNF4α, RXRα and PPARα) and traditional hepatic cells (HepG2-NE, expressing human NTCP).