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Mays Cancer Center at UT Health San Antonio MD Anderson Cancer Center

1 ARTICLES PUBLISHED IN JoVE

Biology

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Bárbara de la Peña Avalos^1,2, Eloïse Dray^1,2

¹Department of Biochemistry and Structural Biology, University of Texas Health Science Center at San Antonio, ²Mays Cancer Center at UT Health San Antonio MD Anderson Cancer Center

Following DNA damage, human cells activate essential repair pathways to restore the integrity of their genome. Here, we describe the method of indirect immunofluorescence as a means to detect DNA repair proteins, analyze their spatial and temporal recruitment, and help interrogate protein-protein interaction at the sites of DNA damage.