Accessing the Cytotoxicity and Cell Response to BiomaterialsAnabela Baptista Paula *1,2,3,4, Mafalda Laranjo *2,3,4,5, Ana Sofia Coelho 1,2,3,4, Ana Margarida Abrantes 2,3,4,5, Ana Cristina Gonçalves 2,3,4,6, Ana Bela Sarmento-Ribeiro 2,3,4,6, Manuel Marques Ferreira 2,3,4,7, Maria Filomena Botelho 2,3,4,5, Carlos Miguel Marto 1,2,3,4,8, Eunice Carrilho 1,2,3,4
1Institute of Integrated Clinical Practice, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, 2Coimbra Institute for Clinical and Biomedical Research (iCBR) area of Environment Genetics and Oncobiology (CIMAGO), University of Coimbra, Coimbra, Portugal, 3Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, Coimbra, Portugal, 4Clinical Academic Center of Coimbra, CACC, Portugal, 5Institute of Biophysics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, 6Laboratory of Oncobiology and Hematology, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, 7Institute of Endodontics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, 8Institute of Experimental Pathology, Faculty of Medicine, University of Coimbra, Coimbra, Portugal
This methodology aims to evaluate biomaterial cytotoxicity through the preparation of soluble extracts, using viability assays and phenotypic analysis, including flow cytometry, RT-PCR, immunocytochemistry, and other cellular and molecular biology techniques.
