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Center for Scientific Research and Higher Education of Ensenada (CICESE)

1 ARTICLES PUBLISHED IN JoVE

Biology

Preparation of Virus-Enriched Inoculum for Oral Infection of Honey Bees (Apis mellifera)

Edward M. Hsieh¹, Jimena Carrillo-Tripp², Adam G. Dolezal¹

¹Department of Entomology, University of Illinois, ²Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada (CICESE)

Here we describe two protocols: first to propagate, extract, purify, and quantify large quantities of honey bee non-enveloped virus particles, including a method for removing honey bee pupae and second to test the effects of viral infection using a highly repeatable, high-throughput cage bioassay.