Preparation of Virus-Enriched Inoculum for Oral Infection of Honey Bees (Apis mellifera)Edward M. Hsieh 1, Jimena Carrillo-Tripp 2, Adam G. Dolezal 1
1Department of Entomology, University of Illinois, 2Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada (CICESE)
Here we describe two protocols: first to propagate, extract, purify, and quantify large quantities of honey bee non-enveloped virus particles, including a method for removing honey bee pupae and second to test the effects of viral infection using a highly repeatable, high-throughput cage bioassay.