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Tsubota Laboratory, Inc.

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Inducement and Evaluation of a Murine Model of Experimental Myopia

Xiaoyan Jiang^1,2, Toshihide Kurihara^1,2, Shin-ichi Ikeda^1,2, Hiromitsu Kunimi^1,2, Kiwako Mori^1,2, Hidemasa Torii^1,2, Kazuo Tsubota²

¹Laboratory of Photobiology, Keio University School of Medicine, ²Department of Ophthalmology, Keio University School of Medicine

In this protocol, we describe the full process of experimental myopia inducement in mice using newly designed eyeglasses and the technic needed for achieving stable and reproducible results in ocular parameter measurements.

Medicine

A Murine Model of Ischemic Retinal Injury Induced by Transient Bilateral Common Carotid Artery Occlusion

Deokho Lee^*1,2, Yukihiro Miwa^*1,2,3, Heonuk Jeong^1,2, Shin-ichi Ikeda^1,2, Yusaku Katada^1,2, Kazuo Tsubota^1,2,4, Toshihide Kurihara^1,2

¹Laboratory of Photobiology, Keio University School of Medicine, ²Department of Ophthalmology, Keio University School of Medicine, ³Animal eye care, Tokyo Animal Eye Clinic, ⁴Tsubota Laboratory, Inc.

Here, we describe a mouse model of retinal ischemia by transient bilateral common carotid artery occlusion using simple sutures and a clamp. This model can be useful for understanding the pathological mechanisms of retinal ischemia caused by cardiovascular abnormalities.