Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron TomographySophie Sosnovskaya *1,2, Amir N. Zakirov *1,2, Ekaterina D. Ryumina *1,3, Ekaterina Kharybina 1,2, Sergei A. Golyshev 1, Olga S. Strelkova 1, Oxana A. Zhironkina 1, Andrei Moiseenko 2, Anton Orekhov 4, Igor I. Kireev 1,2,5
1Belozersky Institute of Physico-chemical Biology, Lomonosov Moscow State University, 2Faculty of Biology, Lomonosov Moscow State University, 3Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 4National Research Center, Kurchatov Institute, 5V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology
This protocol presents a technique for high-resolution mapping of replication sites in structurally preserved chromatin in situ that employs a combination of pre-embedding EdU-streptavidin-Nanogold labeling and ChromEMT.
