Universitat Pompeu Fabra (UPF)
2 ARTICLES PUBLISHED IN JoVE
Developmental Biology
Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers
Frederic Català-Castro 1, Valeria Venturini 2,3, Santiago Ortiz-Vásquez 1, Verena Ruprecht 2,4, Michael Krieg 1
1Neurophotonics and Mechanical Systems Biology, Institut de Ciències Fotòniques, ICFO, 2Center for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, 3Institut de Ciències Fotòniques, ICFO, 4Universitat Pompeu Fabra (UPF)
Here, we present a protocol to investigate the intracellular mechanical properties of isolated embryonic zebrafish cells in three-dimensional confinement with direct force measurement by an optical trap.
Biology
High-Throughput Behavioral Aging and Lifespan Assays Using the Lifespan Machine
Andrea Del Carmen-Fabregat 1,2, Lucia Sedlackova 1, Natasha Oswal 1,2, Nicholas Stroustrup 1,2
1Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology (BIST), 2Universitat Pompeu Fabra (UPF)
The imaging platform "The Lifespan Machine" automates the lifelong observation of large populations. We show the steps required to perform lifespan, stress resistance, pathogenesis, and behavioral aging assays. The quality and scope of the data allow researchers to study interventions in aging despite the presence of biological and environmental variation.
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