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National Academy of Sciences of Ukraine

2 ARTICLES PUBLISHED IN JoVE

Environment

Mistletoe Eradicator - A Novel Tool for Simultaneous Mechanical and Chemical Control of Mistletoe

Khurshid Ahmad Bhat¹, Sabina Akhtar², Naseer Ahmad Dar³, Mohammad Iqbal Bhat³, Farooq Ahmad Bhat¹, Rashid Rizwan⁴, Oleksii Horielov⁵, Yuliya Krasylenko⁶

¹Department of Plant Pathology, FOA Wadura Sopore, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir, ²American University in the Emirates Dubai, ³Krishi Vigyan Kendra, Budgam, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir, ⁴Department of Vegetable Science, FoH, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir, ⁵Department of Dendrology, M.M. Gryshko National Botanical Garden, National Academy of Sciences of Ukraine, ⁶Department of Biotechnology, Palacký University Olomouc

The protocol describes an easy-to-handle tool called Mistletoe Eradicator which combines mechanical and chemical approaches and is efficient in the control of mistletoe infestation on the host trees. The manuscript uses the European mistletoe (Viscum album subsp. album L.) as an example.

Biology

Non-invasive Assay for Chlorophyll Biosynthesis Kinetics Determination during Early Stages of Arabidopsis De-etiolation

Veronika Balakhonova^*1,2, Nadiia Pushkarova^*1,3, Jan Skalak^1,2, Tereza Dobisova^1,2,4, Zuzana Benedikty⁵, Klara Panzarova⁵, Martin Trtilek⁵, Jan Hejátko^1,2

¹CEITEC - Central European Institute of Technology, Masaryk University, ²National Centre for Biomolecular Research, Faculty of Science, Masaryk University, ³Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, ⁴Labdeers s.r.o, ⁵Photon Systems Instruments, Prumyslova

Here, we describe an advanced tool designed for chlorophyll biosynthesis monitoring during the early stages of Arabidopsis seedling de-etiolation. The novel methodology provides non-invasive real-time chlorophyll fluorescence imaging at high spatial and temporal resolution.