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St. Jude Graduate School of Biomedical Sciences

1 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Fixation of Embryonic Mouse Tissue for Cytoneme Analysis

Eric T. Hall¹, Christina A. Daly^1,2, Yan Zhang¹, Miriam E. Dillard¹, Stacey K. Ogden¹

¹Department of Cell and Molecular Biology, St. Jude Children’s Research Hospital, ²St. Jude Graduate School of Biomedical Sciences

Here, an optimized step-by-step protocol is provided for fixation, immunostaining, and sectioning of embryos to detect specialized signaling filopodia called cytonemes in developing mouse tissues.