Fixation of Embryonic Mouse Tissue for Cytoneme AnalysisEric T. Hall 1, Christina A. Daly 1,2, Yan Zhang 1, Miriam E. Dillard 1, Stacey K. Ogden 1
1Department of Cell and Molecular Biology, St. Jude Children’s Research Hospital, 2St. Jude Graduate School of Biomedical Sciences
Here, an optimized step-by-step protocol is provided for fixation, immunostaining, and sectioning of embryos to detect specialized signaling filopodia called cytonemes in developing mouse tissues.
