Simple and Fast Rolling Circle Amplification-Based Detection of Topoisomerase 1 Activity in Crude Biological SamplesJosephine Geertsen Keller 1,2, Karol Mizielinski 3, Kamilla Vandsø Petersen 1,2, Magnus Stougaard 2,3,4, Birgitta R. Knudsen 1,3, Cinzia Tesauro 3
1Department of Molecular Biology and Genetics, Aarhus University, 2Department of Clinical Medicine, Aarhus University, 3VPCIR Biosciences ApS, 4Department of Pathology, Aarhus University Hospital
A protocol for the sensitive and quantitative detection of topoisomerase 1 activity using the rolling circle enhanced enzyme activity detection assay is described. The method allows detection of topoisomerase 1 activity from purified components or cell/tissue extracts. This protocol has wide-ranging applications in any field involving detection of enzymatic activity.
