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University of Colorado-Anschutz School of Medicine

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Analysis of T-cell Receptor-Induced Calcium Influx in Primary Murine T-cells by Full Spectrum Flow Cytometry

Loni Perrenoud¹, James Conley¹, Leslie J. Berg¹

¹Department of Immunology and Microbiology, University of Colorado-Anschutz School of Medicine

Calcium influx, a measure of T-cell signaling, is an effective way to analyze responses to T-cell receptor stimulation. This protocol for multiplexing Indo-1 with panels of antibodies directed at cell surface molecules takes advantage of the highly flexible capabilities of full spectrum flow cytometry.