Analysis of T-cell Receptor-Induced Calcium Influx in Primary Murine T-cells by Full Spectrum Flow CytometryLoni Perrenoud 1, James Conley 1, Leslie J. Berg 1
1Department of Immunology and Microbiology, University of Colorado-Anschutz School of Medicine
Calcium influx, a measure of T-cell signaling, is an effective way to analyze responses to T-cell receptor stimulation. This protocol for multiplexing Indo-1 with panels of antibodies directed at cell surface molecules takes advantage of the highly flexible capabilities of full spectrum flow cytometry.
