Multipronged Phenotyping Approaches to Characterize Sugarcane Root Systems

Resumen

Characterizing root system traits is one of the areas of research that is still in its infancy, particularly in sugarcane. Integrating multiple approaches to precisely phenotype sugarcane roots leads to comprehensive and holistic results, enabling the utilization of identified traits and mechanisms for conventional and molecular breeding.

Resumen

Roots are the primary conductors of water and nutrients and play a vital role in sustaining growth and yield under stressful environments. The study of plant roots poses methodological difficulties in in situ assessment and sampling, which is especially true for sugarcane (Saccharum spp.). Traditional methods during the 1920s documented the genotypic variation in sugarcane root systems, after which few studies were reported on sugarcane root traits per se until recently. In addition to morphology, rhizosphere characteristics, including allelopathic effects and/or affinity for microbial symbiosis, determine plant establishment and survival.

Ultimately, root systems define the above-ground productivity of sugarcane. With the impetus for climate-resilient varieties, it is becoming more relevant to explore and utilize the variability in root system traits of sugarcane. This paper describes multipronged approaches for sugarcane root phenotyping, including field excavation by trench sampling, the use of a root core sampler, raised platforms for root sampling, and raising plants under hydroponic culture, employed by a team of scientists at the Indian Council of Agricultural Research-Sugarcane Breeding Institute (ICAR-SBI).

Field excavation by trench sampling is imperative to assess the plant roots in their natural growing environment. The use of raised platforms simulating field conditions and a root core sampler are alternative approaches, with a considerable reduction in time, uniform sample size, and less loss of root material. Hydroponic plant culture allows the study of morphology, anatomical features, and rhizosphere biology, including the exudation of organic compounds and microbial interactions. Data generated from different experiments using diverse sampling methods add to the wealth of information on the root system traits of sugarcane.

Introducción

Sugarcane (Saccharum spp.), an important food and bioenergy source, is a significant industrial crop suited for cultivation in many countries with tropical and subtropical climatic conditions. Owing to the C₄ photosynthetic pathway for carbon assimilation, sugarcane is highly productive, efficiently using farm inputs such as water and fertilizers. Post-harvest processing of sugarcane yields economically valuable products such as sugar and jaggery, alongside its byproducts-molasses, ethanol, and energy. Sugarcane is produced by nearly 100 countries over an area of 25.97 Mha, which is approximately 1.5% of total arable land. India alone contributes to 16% of world sugarcane production (approximately 306 Mt), with an average productivity of 70 t·ha^{−1 1}. The major abiotic stresses drastically affecting sugarcane production include water deficits, water logging, temperature extremes, and soil properties such as nutrient deficiency, salinity, sodicity, and alkalinity. The biggest challenge for developing abiotic stress-tolerant crops is to pinpoint specific traits that confer a substantial yield advantage under stress conditions.

Several aspects of sugarcane physiology are poorly understood, including the root-shoot relationship, which drastically affects cane productivity. Sugarcane root is not as well studied as shoots, although the different types, such as sett roots, shoot roots, and adult roots, may be developmentally distinct with varying functions. Genotypic differences have been observed with regard to the number and length of sett roots emerging during germination². Sett roots are implicated in the germination of sugarcane buds, ensuring early crop establishment, and are later replaced by shoot roots, which are robust, emerging from the base of the developing shoot³. Fine branches observed in the sett roots help in anchoring the young plants and aid in the absorption of water and nutrients until they are replaced by shoot roots. Similar to sett roots, shoot roots also arise from the root primordia present in the lower, unexpanded internodes of the cane.

As shoot roots persist in the plant for a longer duration, they are 4x-10x thicker than sett roots. Shoot roots constitute the sole root system of sugarcane, with an important role in further growth and development. The vigor of the shoot roots is positively associated with the overall vegetative vigor of the plant. The continuous development of roots resulting from the turnover of sett roots and shoot roots gives rise to the "adult root system" of sugarcane, which is ever-adapting to the prevailing environmental conditions. In general, a deeper, more prolific root system makes more water and nutrients available for the crop than does a shallower distribution of roots. Periodic dissections revealed that, when the soil moisture content was high, shallow root systems were observed, whereas a much deeper root system developed as the water table dropped². The root system in sugarcane remains active even after harvesting of the crop, contributing to the growth of the ratoon crop until new shoot roots emerge from underground buds⁴. Root angle and the level of root branching are two important factors determining the volume of soil explored by plant roots. Root angle, a genotypic trait, may be altered through conventional breeding or molecular approaches to improve tolerance to biotic and abiotic stresses. On the contrary, the level of root branching is mostly influenced by the environment, necessitating periodic monitoring of root development and its response to localized soil conditions.

Anatomical features of sugarcane roots have been examined to ascertain differences with regard to genotype and environment. The anatomy of sett roots in sugarcane resembles that of mature roots in other grasses such as maize, wherein the cortex comprises well-differentiated cell layers in a regular pattern. The endodermis is suberized, followed by a single-layered pericycle. Metaxylem elements are the main conductors or water and nutrient ions, radially arranged and interspersed with groups of phloem, the latter comprising a sieve element with two companion cells. The large central mass of undifferentiated cells forms the root pith. Distinct anatomical features of sugarcane cultivars correspond to root hydraulic properties, thereby influencing water movement. Early studies on the differences in the root anatomical traits of sugarcane revealed that, under low moisture stress conditions, pronounced thickening of the cell wall was observed in the layers immediately inside of the endodermis, between the pith and the vascular region, and around the vessels⁵. Such thickened cells may be an adaptation to prevent the backward flow of sap and for mechanical strength during stress.

Some important traits implicated in the drought resistance of sugarcane include the relative thickness and number of exodermal layers, the ratio of cortex to stele, intercellular spaces in the cortex, and thickened root hair tips. The ratios of the area occupied by cortical cells to the area occupied by the stellar tissues of shoot roots are significantly different among sugarcane cultivars, with wide variability with respect to the area of the stele⁶. The hydraulic conductivity of sugarcane roots is related to the size and number of metaxylem elements in the shoot roots. Hydrophobic cell layers within the root are likely to define zones of apoplastic water movement. Suberized Casparian bands are found in the endodermis and in the hypodermis (termed as exodermis), which serve as hydrophobic barriers. The disintegration of cortical cells leads to the formation of lysigenous aerenchyma in older roots and in plants subjected to hypoxic conditions, irrespective of developmental age. The formation of aerenchyma during waterlogging stress is correlated with the maintenance of growth in resistant varieties⁷.

The morphology and anatomy of Erianthus arundinaceus [Retzius] Jeswiet (genera related to Saccharum spp.) roots are implicated in its strong tolerance to environmental stresses⁸. Erianthus arundinaceus roots exhibit nodal roots distributed at steep angles, with dense roots hairs to facilitate the uptake of water and nutrient ions from deeper soil zones. The deep-root system consists of many nodal roots growing with steep growth angles. The diameter of the nodal roots correlates with the size and number of large xylem vessels, the former varying widely from 0.5 mm to 5.0 mm. These nodal roots also form a rhizomatous sheath, with a hypodermis showing lignified sclerenchyma in the outer cortex (exodermis), lysigenous aerenchyma in the mid-portion of the cortex, and starch granules in the stele. In addition to architecture and morphological traits, root-exuded organic compounds play an important role in determining plant germination, establishment, and survival, with plausible allelopathic effects and/or affinity for microbial symbiosis.

Root enzymatic activity and the finer details of the morphology, including root cap pigmentation and rejuvenation potential upon injury, were documented in sugarcane varieties grown under hydroponic culture⁹. Root growth shows a highly plastic response to changes in the soil environment, both in terms of the form and size of the root system. The most efficient sugarcane variety would be one that has few or an optimal number of shoots, with a correspondingly lower number of roots, aiding better survival during stressful conditions. The systematic study of the root system should, thus, form an important component of any crop improvement program¹⁰. The majority of the experiments focusing on roots rely mostly on developmental aspects, while a focus on functional plasticity is often lacking¹¹. Apart from the structural distribution, functional root plasticity plays a crucial role in survival under stress and would, therefore, support breeders in their efforts to include root system traits in the selection pipeline for abiotic stress tolerance and improve the robustness of sugarcane.

Considering its importance in sustaining growth and yield under stressful environments, it is essential to explore and utilize the inherent variability in root system traits of sugarcane. An emphasis on the selection of component traits and mechanisms imparting superior root systems is the way forward for better crop performance under changing climatic conditions. Phenotypic evaluation is a long and costly process; however, the integration of multipronged approaches would add tremendous value to its utility in crop improvement. In this manuscript, four different approaches for root phenotyping in sugarcane are described, each with its own set of merits and demerits, implying that a concerted effort is required to arrive at comprehensive and holistic results.

Protocolo

1. Field excavation by trench sampling

1. Raise commercial sugarcane hybrids (hereafter called "varieties") in the field from two-budded setts planted at a row spacing of 120 cm, with 90 cm within rows, following the recommended package of practices (POP) to ensure good crop establishment and growth.
2. At the end of the maturity phase of the crop, employ an excavator to dig a trench (1.5 m deep and 1.0 m wide) in the field. Through continuous water jetting, ensure that the soils from root zones are cleared without damaging the roots (Figure 1).
3. When the adhering soil loosens, uproot the cane along with the root system and take it to the laboratory for manual measurement of the number of roots, root length, volume, and weight.

Figure 1: Trench sampling method for the field excavation of roots. (A) Sideways trench dug along the field, (B) water jetting, and (C) inner view showing the depth of the trench. Please click here to view a larger version of this figure.

2. Root core sampler to reduce sampling errors

1. Use a cylindrical root core sampler of 61 cm height and 16 cm diameter weighing 8 kg, fabricated using mild steel (MS) material for sugarcane root sampling in the field. Provide a sharp edge at the bottom edge of the sampler to facilitate easy penetration while inserting it into the soil. Provide the top with collars of 3 cm diameter to lift the sampler (Figure 2).
2. Raise sugarcane varieties in the field from two-budded setts planted at a row spacing of 120 cm, with 90 cm within rows, following the recommended POP to ensure good crop establishment and growth.
3. At the maturity phase of the crop, fasten the top edge of the sampler to the primary shoot/cane and hammer continuously to reach the desired soil depth (45 cm). Lift the entire soil mass into the sampler and wash carefully under running water to separate the adhering roots.
4. After thorough washing of the roots, record the volume, surface area, length, and weight by manual measurement, as well as by spreading the roots on transparent trays to scan and analyze the corresponding digitized images using the referenced software (see the Table of Materials).

Figure 2: Root core sampler. (A) Dimensions, (B) top view, and (C) site of sampling. Please click here to view a larger version of this figure.

3. Root phenotyping structure to facilitate sampling at different phenophases

1. Construct a root phenotyping structure comprising three adjacent compartments of dimensions 4.5 m x 10.1 m for sampling of the sugarcane roots, with provisions for manually dismantling the side walls to reveal the underground root system (up to a depth of 80-100 cm) (Figure 3). Construct the side walls with precast slabs of dimensions 1.8 m in length, 30 cm in width, and 4 cm in thickness.
2. Fill and compact the structure with field soil, leaving a headspace of ~20 cm, with adequate drainage holes to facilitate soil aeration.
3. Sow the bud chips of germplasm clones comprising Saccharum officinarum L., Saccharum spontaneum L., Saccharum barberi Jesw., Saccharum sinense Roxb., and Saccharum robustum Brandes and Jeswiet ex Grassl. (hereafter called "Saccharum spp. clones") and allow them to germinate for 30 days in protrays comprising rooting media (red soil: farm yard manure: sand = 2:2:1). Transplant uniform and healthy settlings to the structure at a row spacing of 90 cm, with 60 cm within rows, following the recommended POP to ensure good crop establishment and growth.
4. During the formative (60-120 days after planting [DAP]) and grand growth (120-150 DAP) phases, manually remove the side walls made of the precast slabs, followed by continuous spraying of the water jet to expose the roots.
5. Uproot the entire root system and take it to the laboratory for manual measurement of the length, volume, and weight, and spread the roots on transparent trays to scan and analyze the corresponding digitized images in the referenced software (see the Table of Materials).
6. Impose drought stress by withholding irrigation in one of the compartments, and plug the drainage holes in the second compartment to maintain soil saturation to simulate waterlogging stress. Irrigate the third compartment according to the recommended POP to maintain the field capacity and to serve as a control.

Figure 3: Root phenotyping structure. (A) Dimensions, (B) overview of the three compartments, and (C) view of one compartment. Please click here to view a larger version of this figure.

4. Hydroponic culture of plants to study rhizosphere biology

1. Fabricate an in-house hydroponic system in an environment-controlled glasshouse conducive for growing sugarcane to study the finer details of root biology. Add ~15 L of modified Hoagland's nutrient solution (Table 1) to glass tanks of dimensions 20 cm x 20 cm x 50 cm, with aeration provided by aquarium pumps (Figure 4).
2. Sow the bud chips of sugarcane varieties and Saccharum spp. clones and allow them to germinate for 30 days in protrays comprising composted coir pith. Transplant uniform and healthy settlings to hydroponic tanks at the frequency of three settlings per tank, taking care to place the entire root in the nutrient solution. Cover the tanks with black cloth to ensure that roots are not exposed to light. Use a plastic mesh (20 cm x 20 cm) at the brim of the glass tanks to support the plants upright.
3. At the end of the germination phase (60 days), collect the root exudates by immersing the roots of intact plants in 50 mL of trap solution (sterile double-distilled water) for 4 h (0800 h to 1200 h) during the time of peak photosynthetic activity. Filter the collected solution through Whatman filter paper, then pass it through glass columns filled with anion-exchange followed by cation-exchange resins¹². Evaporate the eluted fractions to dryness and store at -20 °C until further processing.
4. Analyze the processed root exudate samples by HPLC for the determination of organic acids¹² and by spectrophotometry for the estimation of total phenolics¹³, proteins¹⁴, sugars¹⁵, and amino acids¹⁶ according to standard protocol.
5. Monitor the root growth at weekly intervals to record the root tip pigmentation and root hair density. Assess the activity of the enzymes, peroxidase¹⁷ and superoxide dismutase¹⁸, and total phenolic content¹⁹ at the 3rd month according to standard protocol.
6. Assess the response to root injury by inflicting a longitudinal slice in the primary root up to the root tip using a sterile surgical blade, and monitor the changes periodically.

Chemical	Final concentration
Potassium nitrate	0.608 g·L-1
Calcium nitrate	1.415 g·L-1
Potassium dihydrogen phosphate	0.164 g·L-1
Magnesium sulphate	0.560 g·L-1
EDTA-ferric monosodium salt	6.00 g·250L-1
Boric acid	1.43 g·250L-1
Manganese chloride tetrahydrate	0.91 g·250L-1
Zinc sulphate	0.11 g·250L-1
Cupric suphate	0.04 g·250L-1
Molybdic acid	0.01 g250L-1

Table 1: Composition of the modified nutrient solution for the hydroponic culture of sugarcane.

Figure 4: Hydroponics setup. The setup (A) customized for growing sugarcane and (B) 5-month-old crop (black cloth removed for photography purpose only). This figure has been modified from Hari et al.⁹. Please click here to view a larger version of this figure.

Resultados

Variation in the root morphological traits of sugarcane varieties
Representative images of the root system in Co 62175, excavated from the field by trench sampling and grown in a hydroponic setup, are presented in Figure 5A,B. Long roots (~100 cm) were observed in the varieties Co 62175 and Co 99006, while Co 99006 recorded the highest root weight (127 g·clump⁻¹). Root traits were recorded using a root core sampler, which revealed...

Discusión

Root systems define the above-ground productivity of sugarcane, necessitating that all its facets be explored and understood thoroughly for the development of climate-resilient varieties. A team of scientists at ICAR-SBI comprising plant physiologists, a microbiologist, an agricultural engineer, a biochemist, and plant breeders employed multipronged approaches for sugarcane root phenotyping, including field excavation by trench sampling, the use of a root core sampler, raised platforms for root sampling, and raising plan...

Materiales

Name	Company	Catalog Number	Comments
Aeration pump with pipeline accessories	Purchased from local sources	NA	Used for hydroponic culture of sugarcane
Boric acid	Sisco Research Laboratories, India	80266	Preparation of modified Hoagland's solution
Calcium nitrate	Central Drug House, India	27606	Preparation of modified Hoagland's solution
Composted coir pith	Purchased from local sources	NA	Used for germinating sugarcane setts
Cupric sulphate	Sisco Research Laboratories, India	38869	Preparation of modified Hoagland's solution
DEAE-cellulose	Sisco Research Laboratories, India	10529	anion exchange resin for processing of root exudates
EDTA-ferric monosodium salt	Sisco Research Laboratories, India	59389	Preparation of modified Hoagland's solution
Farm yard manure	Purchased from local sources	NA	Used for germinating sugarcane setts
Glass tanks	Fabricated in-house	NA	Used for hydroponic culture of sugarcane
HPLC	Agilent Technologies	1200 Infinity	Quantification of organic acids in root exudates
Magnesium sulphate	Sisco Research Laboratories, India	29117	Preparation of modified Hoagland's solution
Manganese chloride	Sisco Research Laboratories, India	75113	Preparation of modified Hoagland's solution
Molybdic acid	Sisco Research Laboratories, India	49664	Preparation of modified Hoagland's solution
Potassium dihydrogen phosphate	Central Drug House, India	29608	Preparation of modified Hoagland's solution
Potassium nitrate	Central Drug House, India	29638	Preparation of modified Hoagland's solution
Protrays	Fabricated in-house	NA	Used for germinating sugarcane setts
Red soil	Purchased from local sources	NA	Used for germinating sugarcane setts
Root core sampler	Fabricated in-house	NA	Used for in situ root sampling
Sand	Purchased from local sources	NA	Used for germinating sugarcane setts
Seralite-120	Sisco Research Laboratories, India	14891	cation exchange resin for processing of root exudates
Supporting frame	Purchased from local sources	NA	Used for hydroponic culture of sugarcane
Water motor pump	Purchased from local sources	NA	Used for hydroponic culture of sugarcane
Whatman filter paper grade 1	Universal Scientific	1001090	Processing of root exudates
WinRhizo PRO (software)	Regent Instruments Inc., Canada	STD4800	Two-dimensional root scanner with software for analysis of roots
Zinc sulphate	Sisco Research Laboratories, India	76455	Preparation of modified Hoagland's solution