March 8th, 2010
•This assay assesses the ability of a signaling molecule, here Bone Morphogenetic Protein 7 (BMP7), to reorient commissural axons. An explant of embryonic dorsal spinal cord is cultured adjacent to an aggregate of COS cells secreting the candidate growth factors. Reoriented commissural axons growing within the explant are visualized by immunohistochemistry.
Tags
Videos relacionados
Fabrication of a Microfluidic Device for the Compartmentalization of Neuron Soma and Axons
Spinal Cord Electrophysiology
Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts
Photobleaching Assays (FRAP & FLIP) to Measure Chromatin Protein Dynamics in Living Embryonic Stem Cells
Assaying the Kinase Activity of LRRK2 in vitro
A System for ex vivo Culturing of Embryonic Pancreas
Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development
Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells
Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC (Crosslinking of Small Molecules to Isolate Chromatin)
Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados