Identification of Limbal Niche Cells (LNCs)

To identify the limbal niche cells or LNCs by immunofluorescence staining, add 1 milliliter of 0.25%trypsin EDTA per well to the LNCs cultured in a 5%matrigel coated 6 well plate. Digest the cells by incubating the plate for five minutes at 37 deg