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Isolation of Microvascular Endothelial Tubes from Mouse Resistance Arteries

DOI :

10.3791/50759-v

9:23 min

November 25th, 2013

November 25th, 2013

15,263 Views

1Medical Pharmacology and Physiology, University of Missouri, 2Dalton Cardiovascular Research Center

We present a preparation for visualizing and manipulating calcium signaling in native, intact microvascular endothelium. Endothelial tubes freshly isolated from mouse resistance arteries supplying skeletal muscle retain in vivo morphology and dynamic signaling within and between neighboring cells. Endothelial tubes can be prepared from microvessels of other tissues and organs.

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