Removal of Free c-di-GMP and Other Nucleotides - Soluble Fraction Only
3:08
Pellet Resuspension and Solubilization - Membrane Fraction Only
5:34
Washing Steps and MS Sample Preparation
9:42
Results: Capture Compound Mass Spectrometric Identification of c-di-GMP Effector Proteins from P. aeruginosa
11:30
Conclusion
The ubiquitous second messenger c-di-GMP controls growth and behavior of many bacteria. We have developed a novel Capture Compound Mass Spectrometry based technology to biochemically identify and characterize c-di-GMP binding proteins in virtually any bacterial species.