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JoVE Journal

Chemistry

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Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos

7.9K Views

08:42 min

February 19th, 2016

February 19th, 2016

7,902 Views

0:05

Title

0:33

Embryo Fixation

1:26

Immunostaining Whole Embryos

3:47

Confocal Imaging and Processing

6:37

Results: Immunofluorescence of Phospho-epitopes in Whole Mount Zebrafish Ciliated Organs

8:11

Conclusion

Transcribir

The overall goal of this procedure is to immunolocalize proteins that are activated in the zebrafish embryo. This method can help answer key questions in the developmental and cell biology field, such as the localization and activation of phosphor

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Techniques are described to immunostain phospho-epitopes in whole zebrafish embryos and then conduct two-color fluorescent confocal localization in cellular structures as small as primary cilia. The techniques for fixing and imaging can define the location and kinetics of the appearance or activation of specific proteins.

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