Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical (Methylation) and Physical (Mass Spectrometry, Nuclear Magnetic Resonance) Techniques

0:53

Labeling of the Reducing End (RE) Sugar Residue of W-sol AXs with 2-aminobenzamide (2AB)

2:11

Generation of Xylo-oligosaccharides from 2 AB Labeled W-sol AXs

3:34

Matrix-assisted Laser Desorption Ionization-time of Flight Mass Spectrometry (MALDI-TOF MS)

4:48

Electrospray Ionization Quadrupole Time-of-flight Mass Spectrometry (ESI-QTOF MS)

6:20

ESI-MSⁿ

7:30

H¹ Nuclear Magnetic Resonance (NMR) Spectroscopy

8:45

Results: Reducing End (RE) and Internal Region Glycosyl Sequence(s) of Heteroxylans

10:34

Conclusion