Grafting Amine Functional Groups on Cellulose Paper Discs
3:10
Paper-based ELISA for IgG Detection
6:28
Results: Analysis of the Binding Activity of Immobilized Antibodies to the Covalent Immobilized Cellulose Paper Matrix
8:21
Conclusion
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The overall goal of this methodology is to stabilize antibodies and proteins on a cellulose matrix to facilitate development of a naked-eye colorimetric immunoassay which can be useful for point-of-care diagnostics. This method can help answer key
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In this manuscript, periodate oxidation and glutaraldehyde cross-linking methods for covalent immobilization of antibodies on paper discs are presented. The binding activity of immobilized antibodies was evaluated. Based on these results, a glutaraldehyde cross-linking method was used to develop a novel paper-based immunoassay for immunoglobulin G (IgG) detection.