Placental Aggregation and Colony Forming Unit Assays in Methylcellulose
8:32
Results: Transcription Factor-induced Hemogenic Programming of Mouse Embryonic Fibroblasts
9:58
Conclusion
Transcripción
The overall goal of this hemogenic induction protocol is to instigate a developmental process in mouse fibroblasts through transcription factor overexpression to generate hematopoietic precursor cells in vitro that product hematopoietic cells upon
Sign in or start your free trial to access this content
The protocol described here details the induction of a hemogenic program in mouse embryonic fibroblasts via overexpression of a minimal set of transcription factors. This technology may be translated to the human system to provide platforms for future study of hematopoiesis, hematologic disease, and hematopoietic stem cell transplant.